Peptide Stacking
Protocols
Eight synergistic peptide stacks targeting specific biological pathways — from tissue repair to cognitive enhancement.
Tissue Repair Stack
The tissue repair protocol is designed for athletes, post-injury recovery, and anyone dealing with chronic tendon or ligament damage. BPC-157 acts as the cornerstone healer, modulating growth factor signaling and promoting angiogenesis in damaged tissue. TB-500 provides systemic coverage by upregulating actin, facilitating cell migration and reducing inflammation throughout the body. Ipamorelin pulses growth hormone in a physiologically natural pattern, accelerating collagen synthesis and satellite cell recruitment. Together, these three peptides address healing from multiple angles — local tissue signaling, systemic repair facilitation, and upstream hormonal amplification. This stack is typically run for 8–12 weeks alongside targeted physical therapy.
| Peptide Name | Dosage | Frequency | Role | Notes |
|---|---|---|---|---|
| BPC-157 | 500 mcg/day | 2x daily SC | Primary healer | Split into AM/PM doses; inject near injury site |
| TB-500 | 2.0 mg | 2x/week SC | Systemic repair | Upregulates actin; excellent for tendons |
| Ipamorelin | 200 mcg | 3x/day SC | GH pulse | Administer on empty stomach; no cortisol spike |
Growth Hormone Optimization Stack
The growth hormone stack leverages the synergy between GHRH analogs and GH secretagogues to restore youthful GH pulsatility without suppressing the hypothalamic-pituitary axis. CJC-1295 with DAC extends the GHRH signal over days, providing a sustained baseline elevation. Ipamorelin delivers clean GH pulses without cortisol, prolactin, or ghrelin blunting. MK-677 operates through an oral route, stimulating the ghrelin receptor to increase IGF-1 and GH over 24 hours. This triple approach covers pulsatile, sustained, and receptor-level GH stimulation. Ideal for body composition improvement, recovery acceleration, and age-related GH decline. Protocol length is typically 3–6 months with blood work monitoring.
| Peptide Name | Dosage | Frequency | Role | Notes |
|---|---|---|---|---|
| CJC-1295 | 2 mg | 1x/week SC | GHRH analog | With DAC for extended half-life; blunt GH elevation |
| Ipamorelin | 200 mcg | 3x/day SC | GH pulse | Best at night before bed; selective GH release |
| MK-677 | 25 mg/day | Daily oral | GH secretagogue | Take with food; may increase appetite and water retention |
Post-Surgical Recovery Stack
Post-surgical healing demands simultaneous support for wound closure, immune regulation, and inflammation control. BPC-157 accelerates incision healing and protects gut integrity — particularly important following abdominal procedures or general anesthesia. Thymosin Beta-4 modulates the immune response, reducing excessive inflammation while promoting orderly scar tissue remodeling. KPV, a tripeptide derived from alpha-MSH, provides potent oral anti-inflammatory activity through melanocortin receptor signaling without immunosuppression. This stack is intended to run from day 2 post-surgery through week 6–8. Always coordinate with the surgical care team before initiating. Peptides should not substitute prescribed post-op medications.
| Peptide Name | Dosage | Frequency | Role | Notes |
|---|---|---|---|---|
| BPC-157 | 500 mcg | 2x/day SC | Wound healing | Inject near incision perimeter; protects gut lining |
| Thymosin B4 | 2 mg | 2x/week SC | Immune modulation | Reduces fibrosis; promotes orderly tissue remodeling |
| KPV | 100 mcg | 3x/day oral | Anti-inflammatory | Melanocortin pathway; stable in capsule form |
Metabolic Optimization Stack
This protocol targets visceral adipose tissue, insulin sensitivity, and lipolytic signaling. AOD-9604 is a fragment of hGH (amino acids 176–191) that stimulates fat breakdown without the diabetogenic effects of full-length GH. CJC-1295 at a lower weekly dose maintains anabolic signaling and lean mass preservation during a caloric deficit. Tesamorelin is FDA-approved for HIV-associated lipodystrophy and has robust evidence for preferential reduction of visceral fat through GHRH receptor stimulation. The combined effect is improved body composition without significant appetite or blood sugar disruption. Best results when paired with a structured nutrition plan and resistance training. Monitor IGF-1 and fasting glucose quarterly.
| Peptide Name | Dosage | Frequency | Role | Notes |
|---|---|---|---|---|
| AOD-9604 | 300 mcg/day | Daily SC | Fat loss | hGH fragment 176-191; no effect on blood glucose |
| CJC-1295 | 1 mg | 1x/week SC | GH baseline | Lower dose to preserve lean mass during cut |
| Tesamorelin | 2 mg/day | Daily SC | Visceral fat | FDA-approved GHRH analog; strongest visceral evidence |
Longevity & Healthspan Stack
The longevity protocol addresses the core biological hallmarks of aging: telomere attrition, mitochondrial dysfunction, and cellular energy decline. Epitalon, a tetrapeptide derived from the pineal gland, activates telomerase and has demonstrated life extension in multiple animal models with emerging human observational data. Humanin is a mitochondrially encoded peptide that protects against apoptosis, reduces amyloid toxicity, and supports metabolic efficiency. SS-31 (Elamipretide) concentrates in the inner mitochondrial membrane, reducing reactive oxygen species and restoring ATP production. This stack is run in cycles of 20 days on, 10 days off. Appropriate for adults over 40 focused on healthspan rather than pure performance.
| Peptide Name | Dosage | Frequency | Role | Notes |
|---|---|---|---|---|
| Epitalon | 10 mg/day | Daily SC | Telomere support | 20-day cycles; activates telomerase enzyme |
| Humanin | 2 mg | 3x/week SC | Mitochondrial health | Protects against amyloid; metabolic cytoprotection |
| SS-31 | 3 mg/day | Daily SC | Mito protection | Elamipretide; cardiolipin binding; reduces ROS |
Immune Optimization Stack
This protocol supports innate and adaptive immune function, making it relevant for chronic illness, frequent infections, autoimmune dysregulation, or post-viral recovery. Thymosin Alpha-1 is the gold-standard immune modulator, with decades of clinical use in hepatitis, cancer, and sepsis. It trains T-cell maturation and enhances dendritic cell signaling without overstimulating autoimmune responses. LL-37 is the primary human cathelicidin, functioning as a broad-spectrum antimicrobial peptide while modulating inflammatory cytokines. BPC-157 rounds the stack by restoring gut barrier integrity — the site of 70% of immune system activity. This protocol is especially relevant following antibiotic use, COVID-19, or periods of high physiological stress.
| Peptide Name | Dosage | Frequency | Role | Notes |
|---|---|---|---|---|
| Thymosin A1 | 1.6 mg | 2x/week SC | Immune training | T-cell maturation; used in hepatitis & oncology |
| LL-37 | 1 mg/day | Daily SC | Antimicrobial | Cathelicidin derivative; modulates cytokine storm |
| BPC-157 | 250 mcg | 2x/day SC | Gut/immune axis | Restores gut lining; 70% of immune activity is gut-based |
Cognitive Enhancement Stack
The cognitive stack targets neuroplasticity, BDNF upregulation, anxiolytic modulation, and neurogenesis — key pillars of both peak cognitive performance and long-term brain health. Semax, a synthetic analog of ACTH, markedly increases BDNF and NGF expression while improving focus and working memory. Selank, derived from tuftsin, exerts pronounced anxiolytic effects through the GABAergic system without sedation or dependence, making it ideal for stress-heavy cognitive demands. Dihexa is a potent oral neurogenic compound that directly stimulates HGF/c-Met signaling — a pathway essential for synaptogenesis and memory consolidation. Together these peptides provide nootropic benefit across both acute performance and chronic neuroprotection.
| Peptide Name | Dosage | Frequency | Role | Notes |
|---|---|---|---|---|
| Semax | 300 mcg/day | Daily nasal | BDNF / focus | ACTH analog; increases NGF; delivered intranasally |
| Selank | 300 mcg/day | Daily nasal | Anxiolytic | Tuftsin analog; non-sedating; GABAergic modulation |
| Dihexa | 10 mg/day | Daily oral | Neurogenesis | HGF/c-Met agonist; powerful synaptogenic effects |
Sleep Optimization Stack
Quality sleep is the single highest-leverage intervention for recovery, hormonal balance, and cognitive function. This protocol targets multiple phases and mechanisms of sleep architecture. DSIP (Delta Sleep Inducing Peptide) was originally isolated from rabbit cerebral venous blood during delta-wave sleep, and has been shown to increase slow-wave sleep while normalizing circadian rhythm disruption. Ipamorelin at a low pre-sleep dose generates a synchronized GH pulse during the first sleep cycle — the most anabolically important period of the night. GHRP-6 enhances GH amplitude further while providing a gentle appetite signal that can facilitate earlier, deeper sleep onset. This stack should be administered 15–30 minutes before target sleep time on an empty stomach.
| Peptide Name | Dosage | Frequency | Role | Notes |
|---|---|---|---|---|
| DSIP | 200 mcg | Pre-sleep SC | Delta sleep induction | Normalizes circadian disruption; improves SWS |
| Ipamorelin | 100 mcg | Pre-sleep SC | GH pulse | Low dose for nocturnal GH; no cortisol blunting |
| GHRP-6 | 100 mcg | Pre-sleep SC | GH amplitude | Ghrelin mimetic; slight appetite increase is normal |
Explore Non-Surgical Applications
See how these stacking protocols translate to real-world, non-surgical therapeutic applications.
Non-Surgical Uses →